Yes.
Remove old paste first then repaste. Duron is a relatively cool running
chip though, since you're asking I wonder if you dont' have any paste...
in that case you might be able to get by with just the old paste, possibly
at less than optimal interface and few degress hotter. On the other hand
if the original interface is a pre-applied material, not a gease(y) type
but phase-change then it's more important to replace it.