Logon to the DC and from the Run command see if you can access
\\yourdomain.com\sysvol
If you get Access is Denied check Share permissions on Sysvol
Share Permissions:
Default:
Authenticated Users [Full Control]
Administrator group [Full Control]
Everyone [Read]
You could reapply Default security to the DC:
1. Set environment variables listed in Steps 1-7 for Q256000 and reboot the
DC.
256000 Error Messages After Importing Basicdc.inf into Group Policy
http://support.microsoft.com/?id=256000
After the reboot reapply base security:
2. cd %systemroot%\security\templates
3. secedit /configure /cfg "setup security.inf" /db ss.sdb /log ss.log
/verbose
4. secedit /configure /cfg basicdc.inf /db basicdc.sdb /log basicdc.log
/verbose
5. Remove environment variables and reboot the server.