Try the below...
Dim obj As Object
For Each obj In ActiveSheet.OLEObjects
If TypeName(obj.Object) = "OptionButton" Then
obj.Object.GroupName = obj.Name
obj.Object.Value = True
End If
Next
'If you mean CheckBox then
Dim obj As Object
For Each obj In ActiveSheet.OLEObjects
If TypeName(obj.Object) = "CheckBox" Then obj.Object.Value = True
Next
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